Journal: Cell Transplantation
Article Title: Bone marrow mesenchymal stem cells attenuate pain and modulate peripheral sodium channel activity in a rat model of complex regional pain syndrome type I
doi: 10.1177/09636897251383588
Figure Lengend Snippet: Morphological observation, surface marker characterization, and multipotent differentiation potential of primary rat BMSCs. (a) Morphology of primary rat BMSCs observed under an inverted microscope. (b) Flow cytometry analysis of the surface marker CD105 in third-generation BMSCs using FITC-conjugated anti-CD105 antibody. (c) Flow cytometry analysis of the surface marker CD90 in third-generation BMSCs using PE-conjugated anti-CD90 antibody. (d) Flow cytometry analysis of the surface marker CD44 in third-generation BMSCs using FITC-conjugated anti-CD44 antibody. (e) Flow cytometry analysis of the surface marker CD45 in third-generation BMSCs using PE-conjugated anti-CD45 antibody. (f) Flow cytometry analysis of the surface marker CD11b in third-generation BMSCs using PE-conjugated anti-CD11b antibody. (g) Oil Red O staining for adipogenic differentiation of BMSCs, showing lipid droplet formation. (h) Alizarin Red staining for osteogenic differentiation of BMSCs, showing calcium nodule formation ( n = 3).
Article Snippet: Antibodies for flow cytometry included FITC-CD44, PE-CD45, PE-CD90, PE-CD11b (eBioscience, CA, USA), and FITC-CD105 (Bio-Rad, CA, USA).
Techniques: Marker, Inverted Microscopy, Flow Cytometry, Staining